S3D3 [3D3b]细胞
生长状态： 悬浮生长
是否是肿瘤细胞： 0
物种来源： 小鼠
ATCC Number： HB-8331™
细胞形态： **样
**类型： IgG2a
数量： 大量
运输方式： 冻存运输
S3D3 [3D3b]细胞Designations: S3D3 [3D3b]
Depositors: American Cyanamid Co.
Isotype: IgG2a
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: S3D3 [3D3b]细胞immunoglobulin; monoclonal antibody; against Eimeria tenella sporozoites
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Comments: Animals were immunized with E. tenella sporozoites.
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. S3D3 [3D3b]细胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
recommended serum:ATCC 30-2020
References: 3885: Wong RB, et al. S3D3 [3D3b]细胞Antigens and monoclonal antibodies reactive against sporozoites of Eimeria spp.. US Patent 4,710,377 dated Dec 1 1987
