UC8-1B9细胞
生长状态： 悬浮生长
数量： 大量
是否是肿瘤细胞： 0
物种来源： 仓鼠
细胞形态： **样
**类型： IgG
运输方式： 冻存运输
ATCC Number： CRL-1968™
Designations: UC8-1B9
UC8-1B9细胞Depositors: JA Bluestone
Isotype: IgG
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: UC8-1B9细胞Cricetulus migratorius (B cell); Mus musculus (myeloma) deposited as hamster, Armenian (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against dinitrophenyl (DNP)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
UC8-1B9细胞Comments: Animals were immunized with DNP derivatized KLH (keyhole limpet hemocyanin).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody is useful as a control monoclonal antibody when using other hamster derived hybridomas.
Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. UC8-1B9细胞Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
