S194/5.XXO.BU.1细胞
细胞类型： B**细胞
是否是肿瘤细胞： 0
物种来源： 小鼠
数量： 大量
运输方式： 冻存运输
细胞形态： **样
S194/5.XXO.BU.1细胞**类型： IgA (not secreted); kappa light chain (not secreted)
品系： BALB/c
ATCC Number： TIB-20™
相关**： 多发性骨髓瘤
生长状态： 悬浮生长
Designations: S194/5.XXO.BU.1
Depositors: R Hyman, P Ralph, S Sarkar
Isotype: IgA (not secreted); kappa light chain (not secreted)
Biosafety Level: 1
S194/5.XXO.BU.1细胞Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus
Morphology: lymphoblast
Source: Disease: plasmacytoma; myeloma
Strain: BALB/c
Cell Type: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; not secreted
Permits/Forms: S194/5.XXO.BU.1细胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression: H-2d; PC.1
Comments: Resistant to 0.1 mM 5-bromo-2'-deoxyuridine.
Derived from the S194/5.XXO-1 cell line (ATCC TIB-19).
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium, 90%; horse serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 22522: S194/5.XXO.BU.1细胞Hyman R, et al. Cell-specific antigens and immunoglobulin synthesis of murine myeloma cells and their variants. J. Natl. Cancer Inst. 48: 173-184, 1972. PubMed: 4119881
