2254-62.2细胞
是否是肿瘤细胞： 0
物种来源： 仓鼠
生长状态： 贴壁生长
运输方式： 冻存运输
数量： 大量
器官来源： 肾脏
细胞形态： 成纤维样
ATCC Number： CRL-8544™
Designations: 2254-62.2
Depositors: Genentech, Inc.
2254-62.2细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: hamster
Morphology: fibroblast
Source: Organ: kidney
Permits/Forms: 2254-62.2细胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: This line was derived from BHK-21(c-13) (see ATCC CCL-10).
This line has been used as a host for transformation with expression vectors containing selectable and amplifiable marker DNAs (e.g., Factor VIII).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 100 nM methotrexate, 93%; dialyzed fetal bovine serum, 7%
Temperature: 37.0°C
2254-62.2细胞Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, rinse with fresh 0.25% trypsin, remove trypsin and incubate the flask at 37C until the cells detach (about 5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
References: 3983: Capon DJ, et al. 2254-62.2细胞Preparation of functional human factor VIII in mammalian cells using methotrexate based selection. US Patent 4,965,199 dated Oct 23 1990
