203-7D10细胞
ATCC Number： CRL-2651™
是否是肿瘤细胞： 0
物种来源： 小鼠
细胞形态： **样
**类型： IgG2a; IgG2b
运输方式： 冻存运输
数量： 大量
生长状态： 悬浮生长
Designations: 203-7D10
203-7D10细胞Depositors: National Cancer Institute
Isotype: IgG2a; IgG2b
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension but many cells attach
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against a synthetic v-src oncogene peptide
Permits/Forms: 203-7D10细胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with a synthetic polypeptide with the sequence (C)GSSKSKPKDPSQRRHS corresponding to peptides 2 to 17 of the v-src oncogene.
Spleen cells were fused with Sp2/0 myeloma cells.
In immunoblot screening, the antibody detects a 60 kd protein in the src transformed T2 cell line.
A number of additional proteins are also detected in cell lines and embryo tissues including a 50 kd protein in the urine of cancer patients as well as the amniotic fluid of pregnant patients.
The reactivity of the antibody with the src peptide (2-17) was confirmed by ELISA.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: 203-7D10细胞Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.
Maintain cell density between 2 X 10 exp5 and 2 X 10 exp6 viable cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 21967: Niman HL, Lerner RA. 203-7D10细胞Polypeptide-induced monoclonal receptors to protein ligands. US Patent 5,015,571 dated May 14 1991
