R2-10F6细胞
ATCC Number： CRL-2358™
是否是肿瘤细胞： 0
物种来源： 褐鼠
运输方式： 冻存运输
生长状态： 悬浮生长
数量： 大量
**类型： IgG2b
Designations: R2-10F6 [R2-10F6.3C10.3F9]
Depositors: DH Presky
Isotype: IgG2b
R2-10F6细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma)
Morphology:
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against mouse interleukin 12 (IL-12) p40
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Comments: R2-10F6细胞The hybridoma cell line R2-10F6 [R2-10F6.3C10.3F9] was established by David Presky and Victoria Wilkinson in 1993 and subcloned twice.
This antibody is a useful tool for detection of mouse IL-12p40 by ELISA and Western Blot analysis.
R2-10F6 was formed by the fusion of SP2/0 mouse myeloma cells with splenocytes from an ***** female Lewis rat inoculated with recombinant mouse IL-12 p75.
Propagation: ATCC complete growth medium: Iscove's Modified Dulbecco's Medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 0.05 mM 2-mercaptoethanol and 100 U/ml recombinant human IL-6, 90%; fetal bovine serum, 10%.
R2-10F6细胞Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Alternately, cultures can be established by centrifugation with subsequent resuspension in fresh culture medium.
Establish new cultures at 2 X 10 exp5 viable cells/ml and maintain at between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
References: 34475: Wilkinson VL, et al. Characterization of anti-mouse IL-12 monoclonal antibodies and measurement of mouse IL-12 by ELISA. J. Immunol. Methods 189: 15-24, 1996. R2-10F6细胞PubMed: 8576576
