204-4细胞
数量： 大量
生长状态： 悬浮生长
运输方式： 冻存运输
ATCC Number： HB-185™
细胞形态： **样
**类型： IgG2a; kappa light chain
是否是肿瘤细胞： 0
物种来源： 小鼠
204-4细胞Designations: 204-4
Depositors: BS Prabhakar
Isotype: IgG2a; kappa light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: 204-4细胞immunoglobulin; monoclonal antibody; against coxsackievirus B4 (CB4); neutralizing
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Tumorigenic: Yes
Comments: Animals were immunized with coxsackievirus B4 (JVB strain, ATCC VR 184).
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with a highly conserved epitope on the JVB strain of CB4.
Neutralizes most human isolates of CB4 tested.
204-4细胞Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Related Products: immunogen:ATCC VR-184
References: 1328: Prabhaker BS, et al. 204-4细胞High frequency of antigenic variants among naturally occurring human Coxsackie B4 virus isolates identified by monoclonal antibodies. Nature 300: 374-376, 1982. PubMed: 6183593
1329: Prabhaker BS, et al. Detection of conserved and nonconserved epitopes on Coxsackievirus B4: frequency of antigenic change. Virology 146: 302-306, 1985. PubMed: 2413617
58148: Webb SR, et al. Neutralization epitope diversity of Coxsackievirus B4 isolates detected by monoclonal antibodies. J. Med. Virol. 20: 9-15, 1986. PubMed: 2428930
