YPEN-1细胞
年限： 8 weeks
物种来源： 大鼠
是否是肿瘤细胞： 0
细胞形态： 上皮样
数量： 大量
细胞类型： 其他细胞类型
组织来源： endothelium
品系： Copenhagen
器官来源： 前列腺
运输方式： 冻存运输
生长状态： 贴壁生长
相关**： 正常
YPEN-1细胞ATCC Number： CRL-2222™
Designations: YPEN-1
Depositors: K Yamakazi, K Pienta
Biosafety Level: 2 [Cells contain polyomavirus DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology: epithelial
Source: Organ: prostate
Strain: Copenhagen
Tissue: endothelium
Disease: normal
Cell Type:YPEN-1细胞 immortalized with adenovirus 12 - SV40 virus hybrid (Ad12-SV40)
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Isolation: Isolation date: 1993
Tumorigenic: No
Age: 8 weeks
Gender: male
Comments: The YPEN-1 cell line was originated in 1993 from prostate cells of 8 week old Copenhagen male rats.
Cells were cultured in Endothelium Isolation Medium and immortalized by infection with Adenovirus12 SV40 hybrid virus.
The cells stain positively for but are non-producers of SV40 T-antigen.
YPEN-1 cells demonstrate acetylated low density lipoprotein (Dil-Ac-LDL) uptake as an endothelial marker.
They exhibit positive staining for endothelin and for a monoclonal antibody to rat endothelium (MRC OX-43).
They express Integrin a6 1 and Integrin 3 on their plasma membrane, and demonstrate tube formation in Matrigel.
Propagation: YPEN-1细胞ATCC complete growth medium: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, supplemented with 0.03 mg/ml heparin, 95%; fetal bovine serum, 5%
Temperature: 37.0°C
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37�C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37�C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: YPEN-1细胞Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 26 hrs
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 49801: Yamazaki K, et al. Establishment of immortalized Copenhagen rat prostate endothelial cell lines. In Vivo 9: 421-426, 1995. PubMed: 8900918
