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R1.1细胞

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  • 产品名称:R1.1细胞
  • 产品型号:R1.1
  • 产品展商:HZbscience
  • 产品文档:无相关文档
  • 发布时间:2018-07-20
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简单介绍
R1.1细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。R1.1细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。
产品描述

R1.1细胞

细胞类型: T**细胞

生长状态: 悬浮生长

是否是肿瘤细胞: 0

物种来源: 小鼠

数量: 大量

ATCC Number: TIB-42™

相关**: **瘤

运输方式: 冻存运输

品系: C58/J

细胞形态: **样

R1.1细胞器官来源: 胸腺

Designations: R1.1

Depositors: P Ralph

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus

Morphology: lymphoblast


Source: Organ: thymus

Strain: C58/J

Disease: lymphoma

Cell Type: R1.1细胞T lymphocyte;

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Antigen Expression: H-2k; Thy-1.2; TL

Comments: Established from a spontaneous thymoma.

Sensitive to 0.001 mM cortisol and 10 mcg/ml PHA.

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; horse serum, 10%

Subculturing: R1.1细胞Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 1104: Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304

22247: Hyman R, Stallings V. Characterization of a TL- variant of a homozygous TL+ mouse lymphoma. Immunogenetics 3: 75-84, 1976.

22528: Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. R1.1细胞PubMed: 4542714

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