R1-2细胞
运输方式： 冻存运输
数量： 大量
生长状态： 悬浮生长
细胞形态： **样
**类型： IgG2b
ATCC Number： HB-227™
是否是肿瘤细胞： 0
物种来源： 大鼠
Designations: R1-2
R1-2细胞Depositors: IL Weissman
Isotype: IgG2b
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against LPAM-1 (lymphocyte Peyer's patch HEV adhesion molecule), VLA-4
R1-2细胞Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with the Peyer's patch HEV binding lymphoma line TK1.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody recognizes the alpha subunit of a 160000/130000 dalton cell surface heterodimer expressed on lymphocytes and lymphomas capable of binding to high endothelial venules (HEV) of murine Peyer's patch.
This subunit corresponds to the alpha subunit of the human VLA-4 integrin receptor, and the two are structurally closely related (if not identical).
Propagation: ATCC complete growth medium: R1-2细胞RPMI 1640 medium with 0.05 mM 2-mercaptoethanol and 2 mM L-glutamine, 90%; fetal bovine serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1794: Holzmann B, et al. Identification of a murine Peyer's patch-specific lymphocyte homing receptor as an integrin molecule with an alpha chain homologous to human VLA-4alpha. Cell 56: 37-46, 1989. R1-2细胞PubMed: 2463092
