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YI 328-18细胞

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  • 产品名称:YI 328-18细胞
  • 产品型号:YI 328-18
  • 产品展商:HZbscience
  • 产品文档:无相关文档
  • 发布时间:2018-07-06
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简单介绍
YI 328-18细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。YI 328-18细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。
产品描述

YI 328-18细胞

是否是肿瘤细胞: 0

物种来源: 小鼠

生长状态: 悬浮生长

细胞形态: **样

**类型: IgG3

ATCC Number: HB-9306™

运输方式: 冻存运输

数量: 大量

Designations: YI 328-18

Depositors: R Kannagi

YI 328-18细胞Isotype: IgG3

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma)

Morphology: lymphoblast


Cellular Products: immunoglobulin; monoclonal antibody; against glycosphingolipid

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. YI 328-18细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with a synthetic lactoganglioseries glycolipid.

Spleen cells were fused with P3X63Ag8U.1 mouse myeloma cells.

Both YI328-18 (ATCC HB-9306)and YI328-51 (ATCC HB-9307) specifically recognize the abnormal branching structure of the lactoganglioseries glycolipid antigen molecule and have almost no cross-reactivity with smaller unbranched segments.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Medium Renewal: Every 2 days

YI 328-18细胞Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.

Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.

Preservation: Freeze medium: complete growth medium supplemented with an additional 10% fetal bovine serum and 10% (v/v) DMSO.

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 44223: Kannagi R, et al. YI 328-18细胞Monoclonal antibodies specific for synthetic glycosphingolipids having abnormal branched structure. US Patent 4,885,358 dated Dec 5 1989

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