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GSML细胞

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  • 产品名称:GSML细胞
  • 产品型号:GSML
  • 产品展商:HZbscience
  • 产品文档:无相关文档
  • 发布时间:2018-08-23
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简单介绍
GSML细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。GSML细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。
产品描述

GSML细胞

生长状态: 悬浮生长

数量: 大量

ATCC Number: CRL-2699™

运输方式: 冻存运输

组织来源: mononuclear cells

细胞形态: **样

细胞类型: 其他细胞类型

器官来源: 外周血

是否是肿瘤细胞: 0

物种来源: 其他

GSML细胞Designations: GSML

Depositors: JG Scammell

Biosafety Level: 2 [Cells contain Epstein Barr viral DNA sequences ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Saimiri sciureus sciureus

Morphology: lymphoblast


Source: Organ: peripheral blood

Tissue: mononuclear cells

Cell Type: B lymphoblast; immortalized with Epstein-Barr virus (EBV)Epstein-Barr virus (EBV) transformed

Permits/Forms:GSML细胞 In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Gender: male

Comments: GSML is a B lymphocyte cell line established in 2000 by transformation of mononuclear cells from male squirrel monkeys with Epstein-Barr virus derived from B95-8 cells (ATCC CRL-1612).

The cells exhibit elevated expression of the FK506-binding immunophilin FKBP51. [56358]

FKBP51 is the common cause of glucocorticoid resistance.

Chromosome analysis of GSML cells confirm that they are from Guyanese squirrel monkey. [56357]

The Guyanese squirrel monkey and the Bolivian squirrel monkey differ in response to pathologic and pharmacologic stimuli.

Propagation: GSML细胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 X 10 exp5 viable cells/ml. Maintain cell density between 3 X 10 exp5 and 3 X 10 exp6 viable cells/ml.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 24 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:GSML细胞ATCC 30-2020

References: 56357: Scammell JG, et al. The origin of four squirrel monkey cell lines established by karyotype analysis. Cytogenet. Cell Genet. 93: 263-264, 2001. PubMed: 11528123

56358: Scammell JG, et al. Overexpression of the FK506-binding immunophilin FKBP51 is the common cause of glucocorticoid resistance in three New World primates. Gen. Comp. Endocrinol. 124: 152-165, 2001. PubMed: 11703081

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