2E.6细胞
是否是肿瘤细胞： 0
物种来源： 大鼠
数量： 大量
细胞形态： **样
**类型： IgG1
运输方式： 冻存运输
ATCC Number： HB-128™
生长状态： 悬浮生长
Designations: 2E.6
Depositors: JM Davie
2E.6细胞Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse 2E.6细胞(myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against mouse IgG3
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Comments: 2E.6细胞Animals were immunized with mouse myeloma proteins of the IgG1 (MOPC-21), IgG2a (HOPC-1), IgG2b (MPC-11), IgG3 (J606 and GAC-39) and IgM (M104E) subclasses.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with non-essential amino acids, 85%; horse serum, 10%; fetal bovine serum, 5%. If desired, the fetal bovine serum may be omitted
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. 2E.6细胞Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
