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2C5-6细胞

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  • 产品名称:2C5-6细胞
  • 产品型号:2C5-6
  • 产品展商:HZbscience
  • 产品文档:无相关文档
  • 发布时间:2018-06-28
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简单介绍
2C5-6细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。2C5-6细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。
产品描述

2C5-6细胞

运输方式: 冻存运输

是否是肿瘤细胞: 0

物种来源: 小鼠

生长状态: 悬浮生长

数量: 大量

ATCC Number: HB-8995™

细胞形态: **样

**类型: IgM

Designations: 2C5-6

Depositors: G Schwartz

2C5-6细胞Isotype: IgM

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against cholesterol

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. 2C5-6细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with liposomes containing high amounts of cholesterol and lipid A as an adjuvant. [57615]

Spleen cells were fused with P3X63Ag8 myeloma cells.

The antibody detects cholesterol in liposomes containing 71% cholesterol but not with liposomes containing 43% cholesterol. It reacts with crystalline cholesterol in a solid-phase enzyme-linked immunosorbent assay. [57615]

The antibody is capable of detecting cholesterol in situ in the membranes of skeletal muscle sections.

A feeder layer of mouse peritoneal macrophages or mouse fibroblasts (see ATCC 56-X, irradiated STO cells) is require to propagate this cell line.

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose supplemented with 0.1 mM hypoxanthine and 0.016 mM thymidine, 80%; fetal bovine serum, 20%

Temperature: 37.0°C

Subculturing: 2C5-6细胞Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.

Maintain cell density between 2 X 10 exp5 and 1 X 10 exp6 viable cells/ml.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

feeder layer cells:ATCC 56-X

References: 49063: Clarke MS, et al. In situ localization of cholesterol in skeletal muscle by use of a monoclonal antibody. J. Appl. Physiol. 89: 731-741, 2000. PubMed: 10926660

57615: Swartz GM Jr., et al. Antibodies to cholesterol. Proc. Natl. 2C5-6细胞Acad. Sci. USA 85: 1902-1906, 1988. PubMed: 3162316

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