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Y3-Ag 1.2.3细胞

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  • 产品名称:Y3-Ag 1.2.3细胞
  • 产品型号:Y3-Ag 1.2.3
  • 产品展商:HZbscience
  • 产品文档:无相关文档
  • 发布时间:2018-07-28
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简单介绍
Y3-Ag 1.2.3细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。Y3-Ag 1.2.3细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。
产品描述

Y3-Ag 1.2.3细胞

品系: Lou

ATCC Number: CRL-1631™

相关**: 多发性骨髓瘤

数量: 大量

细胞类型: B**细胞

是否是肿瘤细胞: 0

物种来源: 大鼠

生长状态: 悬浮生长

运输方式: 冻存运输

细胞形态: **样

**类型: kappa light chain

Y3-Ag 1.2.3细胞Designations: Y3-Ag 1.2.3

Depositors: K Yamada

Isotype: kappa light chain

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Rattus norvegicus deposited as Rattus sp.

Morphology: lymphoblast


Source: Disease: plasmacytoma; myeloma

Strain: Lou

Cell Type: B lymphoblast;

Cellular Products: immunoglobulin

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Y3-Ag 1.2.3细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: the depositor has provided these cells for research purposes only and with the following restrictions: (1) no distribution should be made to third parties, (2) any proposed commercial use must be negotiated with the National Research Development Corporation, 101 Newington Causeway, London SE1 6BU, (3) all papers reporting any use of these or derived clones should make direct reference to the original publication (Nature 277:131-133, 1979)

Comments: This is a derivative of the S210 myeloma developed by C. Milstein.

No commercial use or third party distribution is permitted without the concurrence of C. Milstein.

The cells are resistant to 8-azaguanine, are HAT sensitive and can be used as a fusion partner for rat B cells to make rat - rat hybridomas.

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Y3-Ag 1.2.3细胞Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.

Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 1090: Galfre G, et al. Rat x rat hybrid myelomas and a monoclonal anti-Fd portion of mouse IgG. Nature 277: 131-133, 1979. PubMed: 310519

26115: Bazin H, et al. Transplantable immunoglobulin-secreting tumours in rats. I. General features of LOU-Ws1 strain rat immunocytomas and their monoclonal proteins. Int. J. Cancer 10: 568-580, 1972.Y3-Ag 1.2.3细胞 PubMed: 4668496

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