GS-109-IV-8细胞
器官来源： 皮肤
细胞形态： 成纤维样
运输方式： 冻存运输
是否是肿瘤细胞： 0
物种来源： 人
生长状态： 贴壁生长
年限： 48 years
ATCC Number： CRL-1672™
相关**： 加德纳综合征
数量： 大量
GS-109-IV-8细胞Designations: GS-109-IV-8
Depositors: EJ Gardner
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: skin
Disease: Gardner's syndrome
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. GS-109-IV-8细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 48 years
Gender: female
Ethnicity: Caucasian
Comments: The tissue donor suffered from Gardner's syndrome.
The cells are said to exist in an initiated state as opposed to a normal or transformed state.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. GS-109-IV-8细胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes). GS-109-IV-8细胞Add fresh medium, aspirate and dispense into new flasks.
