Tu To细胞
器官来源： 皮肤
生长状态： 贴壁生长
细胞形态： 成纤维样
数量： 大量
是否是肿瘤细胞： 0
物种来源： 人
ATCC Number： CRL-1298™
相关**： 其他**
年限： 3 days
运输方式： 冻存运输
Tu To细胞Designations: Tu To
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: skin
Disease: osteogenesis imperfecta (severe congenita)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Tu To细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 3 days
Gender: male
Comments: The tissue donor suffered from osteogenesis imperfecta (congenita, severe).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog Tu To细胞No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Remove spent medium, add fresh 0.25% trypsin for 1 to 3 minutes, remove trypsin and let the culture sit at room temperature for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Tu To细胞Subculture every 6 to 8 days.
