2D10细胞
细胞形态： **样
**类型： IgG2b
年限： *****
ATCC Number： CRL-2226™
品系： Lewis
生长状态： 悬浮生长
数量： 大量
运输方式： 冻存运输
器官来源： 其他
是否是肿瘤细胞： 0
2D10细胞物种来源： 大鼠
Designations: 2D10
Depositors: SE Buchholz
Isotype: IgG2b
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Organ: lymph node, popliteal
Strain: Lewis
Cell Type: hybridoma: B lymphocyte;
Cellular Products: 2D10细胞immunoglobulin; monoclonal antibody; against mouse B7.2
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: *****
Comments: Animals were immunized in the hind footpad with irradiated 5C2 mouse B cell lymphoma cells that had been cultured in the presence of dibutyryl-cAMP.
Popliteal lymph node cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody will neutralize the B7.2 antigen, but does not react with the B7.1 antigen.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium:
0.05 mM 2-mercaptoethanol
fetal bovine serum to a final concentration of 10%
Temperature: 37.0°C
Subculturing: 2D10细胞Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 0.5 X 10 exp5 to 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22286: Powers GD, et al. Expression and functional analysis of murine B7 delineated by a novel monoclonal antibody. Cell. Immunol. 153: 298-311, 1994. PubMed: 7509723
22935: Nabavi N, et al. Signalling through the MHC class II cytoplasmic domain is required for antigen presentation and induces B7 expression. Nature 360: 266-268, 1992. PubMed: 1279442
23485: Chen C, et al. Monoclonal antibody 2D10 recognizes a novel T cell costimulatory molecule on activated murine B lymphocytes. J. Immunol. 152: 2105-2114, 1994. 2D10细胞PubMed: 7510738
