2D-7细胞
生长状态： 悬浮生长
运输方式： 冻存运输
细胞形态： **样
是否是肿瘤细胞： 0
物种来源： 小鼠
**类型： IgG1
数量： 大量
ATCC Number： HB-9667™
Designations: 2D-7
Depositors: Johns Hopkins University
2D-7细胞Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against human centromere protein B (CENP-B)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. 2D-7细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with human CENP-B peptide.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with a major epitope on human CENP-B.
The binding of the 2D-7 antibody is at least partially blocked by sera from patients with anticentromere antibodies.
2D-7细胞Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 22021: Earnshaw W, et al. Cloned human centromere autoantigen. 2D-7细胞US Patent 5,196,307 dated Mar 23 1993
