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GS-109-V-20细胞

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  • 产品名称:GS-109-V-20细胞
  • 产品型号:GS-109-V-20
  • 产品展商:HZbscience
  • 产品文档:无相关文档
  • 发布时间:2018-09-11
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简单介绍
GS-109-V-20细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。GS-109-V-20细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。
产品描述

GS-109-V-20细胞

数量: 大量

生长状态: 贴壁生长

运输方式: 冻存运输

细胞形态: 成纤维样

器官来源: 皮肤

年限: 18 years

是否是肿瘤细胞: 0

物种来源: 人

ATCC Number: CRL-1610™

相关**: 加德纳综合征

GS-109-V-20细胞Designations: GS-109-V-20

Depositors: EJ Gardner

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast


Source: Organ: skin

Disease: Gardner's syndrome

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. GS-109-V-20细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: No

Cytogenetic Analysis: This is a human cell line with the normal diploid 46,XY karyotype. There was no chromosome marker in all 15 metaphases karyotyped. One cell had 47,XY,+7, which may represent a minor subclone occurring at a very low frequency. The Y chromosome was detected in the QM-stained fluorescent microscopy.

Age: 18 years

Gender: male

Ethnicity: Caucasian

Comments: The cells exist in an initiated state as opposed to a normal or a transformed state.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol:

GS-109-V-20细胞Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37�C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37�C.


Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium GS-109-V-20细胞(without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

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