Gr431细胞
数量： 大量
运输方式： 冻存运输
细胞形态： **样
**类型： IgM
ATCC Number： HB-8575™
生长状态： 悬浮生长
是否是肿瘤细胞： 0
物种来源： 人
Designations: Gr431
Gr431细胞Depositors: Sloan-Kettering Institute for Cancer Research
Isotype: IgM
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: human (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: Gr431细胞immunoglobulin; monoclonal antibody; against an intracellular antigen expressed in human malignant cells (cancer, tumor)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Lymphocytes were obtained from the regional lymph nodes of a patient who had breast cancer.
Lymphocytes were fused with NS-1 myeloma cells.
Gr431细胞Propagation: ATCC complete growth medium: RPMI 1640 medium with 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; fetal bovine serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Culture medium, 90%; DMSO, 10%
References: 3897: Thomson TM, et al.Gr431细胞 Human monoclonal antibodies to cell surface antigens. US Patent 4,695,538 dated Sep 22 1987
