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Y-3细胞

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  • 产品名称:Y-3细胞
  • 产品型号:Y-3
  • 产品展商:HZbscience
  • 产品文档:无相关文档
  • 发布时间:2016-09-30
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简单介绍
Y-3细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。Y-3细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。
产品描述

Y-3细胞

细胞形态: **样

**类型: IgG2b; kappa light chain

生长状态: 悬浮生长

ATCC Number: HB-176™

是否是肿瘤细胞: 0

物种来源: 小鼠

数量: 大量

运输方式: 冻存运输

Designations: Y-3

Y-3细胞Depositors: C Janeway

Isotype: IgG2b; kappa light chain

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against H-2 K b

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Y-3细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with Con A stimulated spleen cells from BALB.B mice.

Spleen cells were fused with Sp2/0 myeloma cells.

The antibody cross-reacts with the k, p, q, r and s H-2 haplotypes.

Tested and found negative for ectromelia virus (mousepox).

Y-3细胞Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 1308: Hammerling GJ, et al. Localization of allodeterminants on H-2Kb antigens determined with monoclonal antibodies and H-2 mutant mice. Proc. Natl. Acad. Sci. USA 79: 4737-4741, 1982. Y-3细胞PubMed: 6181513

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