Y-3P细胞
运输方式： 冻存运输
是否是肿瘤细胞： 0
物种来源： 小鼠
数量： 大量
生长状态： 悬浮生长
ATCC Number： HB-183™
细胞形态： **样
**类型： IgG2a
Designations: Y-3P
Depositors: C Janeway
Y-3P细胞Isotype: IgG2a
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:Y-3P细胞 immunoglobulin; monoclonal antibody; against I-A; cytotoxic
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Tumorigenic: Yes
Comments: Animals were immunized with spleen cells from (BALB/c X C57BL/6)F1 mice and boosted with spleen cells from BALB/c mice immunized with either BALB.B or C57BL/6 T cell blasts.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody reacts with I-A b, f, p, q, r, s, u, and v haplotypes and weakly with I-A k.
Y-3P细胞It cross-reacts with all rat I-A like molecules.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1312: Janeway CA Jr., et al. Monoclonal antibodies specific for Ia glycoproteins raised by immunization with activated T cells: possible role of T cellbound Ia antigens as targets of immunoregulatory T cells. J. Immunol. 132: 662-667, 1984.Y-3P细胞 PubMed: 6228596
