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2E6细胞

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  • 产品名称:2E6细胞
  • 产品型号:2E6
  • 产品展商:HZbscience
  • 产品文档:无相关文档
  • 发布时间:2018-06-20
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简单介绍
2E6细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。2E6细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。
产品描述

2E6细胞

生长状态: 悬浮生长

运输方式: 冻存运输

细胞形态: **样

**类型: IgG

ATCC Number: HB-226™

数量: 大量

是否是肿瘤细胞: 0

物种来源: 仓鼠

Designations: 2E6

Depositors: RM Steinman

2E6细胞Isotype: IgG

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Cricetulus migratorius (B cell); Mus musculus (myeloma) deposited as hamster, Armenian (B cell); mouse (myeloma)

Morphology: lymphoblast


Source: Cell Type: hybridoma:lymphoblast B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against mouse beta 2 leukocyte integrins (CD18)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: 2E6细胞Animals were immunized with mouse dendritic cells.

Spleen cells were fused with Sp2/0 myeloma cells.

The antibody reacts precipitates a 150000/90000 dalton CD11/CD18 heterodimer (see also ATCC HB-225) from murine leukocytes.

2E6 blocks the binding function of LFA-1 (CD11a) and CR3/Mac-1 (CD11b).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:

fetal bovine serum to a final concentration of 10%

0.05 mM 2-mercaptoethanol


Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10 exp5 viable cells/ml.Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.

2E6细胞Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 1793: Metlay JP, et al. The distinct leukocyte integrins of mouse spleen dendritic cells as identified with new hamster monoclonal antibodies. J. Exp. Med. 171: 1753-1771, 1990. 2E6细胞PubMed: 2185332

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