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GR-96细胞

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  • 产品名称:GR-96细胞
  • 产品型号:GR-96
  • 产品展商:HZbscience
  • 产品文档:无相关文档
  • 发布时间:2018-08-05
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简单介绍
GR-96细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。GR-96细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。
产品描述

GR-96细胞

生长状态: 悬浮生长

是否是肿瘤细胞: 0

物种来源: 大鼠

ATCC Number: CRL-2013™

数量: 大量

细胞形态: **样

**类型: IgA; kappa light chain

运输方式: 冻存运输

Designations: GR-96

Depositors: SW Russell

Isotype: IgA; kappa light chain

GR-96细胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)

Morphology: lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against mouse receptor for gamma interferon

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: GR-96细胞Animals were immunized with mouse gamma interferon receptor protein purified from solubilized WEHI-3 cells (see ATCC TIB-68).

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

The antibody reacts with an extracellular epitope on the mouse receptor for gamma interferon.

It does not have blocking activity for interferon - receptor binding.

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, supplemented with 0.1mM nonessential amino acids and 15 mM HEPES, 90%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

GR-96细胞Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: Culture medium, 95%; DMSO, 5%

References: 22141: Basu M, et al. A monoclonal antibody against the ligand binding site of the receptor for mouse interferon-gamma. J. Interferon Res. 9: 551-562, 1989. PubMed: 2477471

22295: Basu M, et al. Purification and partial characterization of a receptor protein for mouse interferon gamma. Proc. Natl. Acad. Sci. USA 85: 6282-6286, 1988. PubMed: 2970637

22785: LeClaire RD, et al. Characterization and use of monoclonal and polyclonal antibodies against the mouse interferon-gamma receptor. J. Leukocyte Biol. 51: 507-516, 1992. GR-96细胞PubMed: 1376354

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