2H6-C2细胞
ATCC Number： CRL-1853™
数量： 大量
细胞形态： **样
**类型： IgG2b
生长状态： 悬浮生长
运输方式： 冻存运输
是否是肿瘤细胞： 0
物种来源： 小鼠
Designations: 2H6-C2
2H6-C2细胞Depositors: CA Whetstone
Isotype: IgG2b
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
2H6-C2细胞Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against Bovine herpesvirus 1 (BHV-1, also known as infectious bovine rhinotracheitis virus (IBRV))
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Tumorigenic: Yes
Comments: Animals were immunized with BHV-1.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
2H6-C2细胞The antibody is specific for the gD glycoprotein of BHV-1 and is used by the National Veterinary Services Laboratories of the USDA for in vitro potency testing of adjuvantated, inactivated BHV-1 vaccines.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 92.5%; horse serum, 5%; fetal bovine serum, 2.5%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 24382: . . Conf. Res. 2H6-C2细胞Workers Anim. Dis. : 54, 1987.
