Y-17细胞
生长状态： 悬浮生长
ATCC Number： HB-179™
运输方式： 冻存运输
数量： 大量
是否是肿瘤细胞： 0
物种来源： 小鼠
细胞形态： **样
**类型： IgG2b
Designations: Y-17
Depositors: C Janeway
Y-17细胞Isotype: IgG2b
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against I-E; cytotoxic
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Comments: Animals were immunized with Con A stimulated spleen cells from B10.A(5R) mice.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody reacts with a conformational or combinatorial determinant on Ea:Eb complexes (Ae:Ea) of the b, k, r, s and v haplotypes.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%
Subculturing: Y-17细胞Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
References: 1313: Lerner EA, et al. Monoclonal antibody against an Ir gene product. J. Exp. Y-17细胞Med. 152: 1085-1101, 1980. PubMed: 6158546
