GR-20细胞
运输方式： 冻存运输
是否是肿瘤细胞： 0
物种来源： 大鼠
数量： 大量
生长状态： 悬浮生长
细胞形态： **样
**类型： IgG2a; kappa light chain
ATCC Number： CRL-2024™
Designations: GR-20
GR-20细胞Depositors: SW Russell
Isotype: IgG2a; kappa light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against mouse receptor for gamma interferon
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. GR-20细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with mouse gamma interferon receptor protein purified from solubilized WEHI-3 cells (see ATCC TIB-68).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with an extracellular epitope on the binding site of the mouse receptor for gamma interferon with an avidity of 3.33 X 10 exp-3 l/mol.
It blocks both interferon - receptor binding, and induction of macrophage mediated tumor cell killing.
It does not have agonist activity in the induction of macrophage killing.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. GR-20细胞To make the complete growth medium, add the following components to the base medium:
0.05 mM 2-mercaptoethanol
fetal bovine serum to a final concentration of 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 22141: Basu M, et al. A monoclonal antibody against the ligand binding site of the receptor for mouse interferon-gamma. J. Interferon Res. 9: 551-562, 1989. PubMed: 2477471
22295: Basu M, et al. Purification and partial characterization of a receptor protein for mouse interferon gamma. Proc. Natl. Acad. Sci. USA 85: 6282-6286, 1988. PubMed: 2970637
22785: LeClaire RD, et al. Characterization and use of monoclonal and polyclonal antibodies against the mouse interferon-gamma receptor. J. Leukocyte Biol. 51: 507-516, 1992. GR-20细胞PubMed: 1376354
