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TS2/4.1.1细胞

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  • 产品名称:TS2/4.1.1细胞
  • 产品型号:TS2/4.1.1
  • 产品展商:HZbscience
  • 产品文档:无相关文档
  • 发布时间:2018-07-15
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简单介绍
TS2/4.1.1细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。TS2/4.1.1细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。
产品描述

TS2/4.1.1细胞

ATCC Number: HB-244™

生长状态: 悬浮生长

细胞形态: **样

**类型: mouse IgG1

是否是肿瘤细胞: 0

物种来源: 小鼠

器官来源: 脾

运输方式: 冻存运输

数量: 大量

Designations: TS2/4.1.1

Depositors: TA Springer

TS2/4.1.1细胞Isotype: mouse IgG1

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast


Source: Organ: spleen

Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against lymphocyte function antigen 1 (LFA-1) alpha subunit (CD11a)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. TS2/4.1.1细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: Please acknowledge the origin of this cell line in all relevant publications by citing the following publication(s): [1616]

Comments: Animals were immunized with human cytolytic T lymphocytes.

Spleen cells were fused with NS-1 myeloma cells.

The antibody does not inhibit LFA-1 activity, and can be used to purify fuctionally active LFA-1.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: TS2/4.1.1细胞Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml.

Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 1616: Sanchez-Madrid F, et al. Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3. Proc. Natl. Acad. Sci. USA 79: 7489-7493, 1982. PubMed: 6984191

22361: Dustin ML, et al. TS2/4.1.1细胞Regulation of locomotion and cell-cell contact area by the LFA-1 and ICAM-1 adhesion receptors. J. Immunol. 148: 2654-2663, 1992. PubMed: 1349320

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