2F-2B细胞
品系： C3H/HeJ
ATCC Number： CRL-2168™
数量： 大量
组织来源： vascular epithelium
细胞形态： 上皮样
细胞类型： SV40转化细胞
是否是肿瘤细胞： 0
物种来源： 小鼠
生长状态： 贴壁生长
器官来源： 腋窝**结
运输方式： 冻存运输
2F-2B细胞年限： *****
Designations: 2F-2B
Depositors: KA O'Connell
Biosafety Level: 2 [CELLS CONTAIN PAPOVAVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus
Morphology: epithelial
Source: Organ: axillary lymph node
Strain: C3H/HeJ
Tissue: vascular epithelium
Cell Type: 2F-2B细胞endothelialSV40 transformed
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Tumorigenic: Yes
Antigen Expression: H-2 K; VCAM
Age: *****
Gender: male
Comments: The 2F-2B cell line was derived from a nodular cutaneous solid tumor in nude mice injected with SVEC4-10EE2 (see ATCC CRL- 2167). The line was cloned in 1991 by limiting dilution. The cells are sensitive in vitro to both macrophages and to natural killer (NK) cells.They express the cell surface major histocompatibility complex class I antigen, H-2 k, of the parental cell line, and vascular cell adhesion molecule (VCAM).The cells stain positively for SV40 T antigen.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; heat-inactivated fetal bovine serum, 10%
2F-2B细胞Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Twice per week
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin.
Add fresh trypsin solution (1 to 2 ml), and let the culture sit at room temperature until the cells detach.
Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22840: O'Connell KA, Edidin M. A mouse lymphoid endothelial cell line immortalized by simian virus 40 binds lymphocytes and retains functional characteristics of normal endothelial cells. J. Immunol. 144: 521-525, 1990. PubMed: 2153170
23172: O'Connell KA, Rudmann AA. Cloned spindle and epithelioid cells from murine Kaposi's sarcoma-like tumors are of endothelial origin. J. Invest. Dermatol. 100: 742-745, 1993. PubMed: 8496612
23187: O'Connell K, et al. 2F-2B细胞Endothelial cells transformed by SV40 T antigen cause Kaposi's sarcomalike tumors in nude mice. Am. J. Pathol. 139: 743-749, 1991. PubMed: 1928299
