上海沪震实业有限公司
扫一扫,手机逛起来
主营:ELISA试剂盒,人ELISA试剂盒,小鼠ELISA试剂盒,大鼠ELISA试剂盒,重组蛋白,金标试剂盒,**组化试剂盒,标准品,单克隆抗体,多克隆抗体,生化试剂,生物培养基,原代细胞,细胞株,实验室耗材,动物血清等产品
021-60345367
您现在的位置:
企业信息
9
  • 注册时间:2015-11-06
  • 联系人:游艳
  • 电话:021-60345367
  • 联系时,请说明仪表网看到的
  • Email:shhzsw01@163.com
在线询价
产品详情

GMMe [EPI]细胞

  • 如果您对该产品感兴趣的话,可以
  • 产品名称:GMMe [EPI]细胞
  • 产品型号:GMMe [EPI]
  • 产品展商:HZbscience
  • 产品文档:无相关文档
  • 发布时间:2018-08-19
  • 在线询价
简单介绍
GMMe [EPI]细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。GMMe [EPI]细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。
产品描述

GMMe [EPI]细胞

年限: *****

是否是肿瘤细胞: 0

物种来源: 其他

器官来源: **

生长状态: 贴壁生长

细胞形态: 上皮样

ATCC Number: CRL-2674™

运输方式: 冻存运输

数量: 大量

组织来源: endometrium

Designations: GMMe [EPI]

GMMe [EPI]细胞Depositors: BD Murphy

Biosafety Level: 2 [Cells contain SV40 viral DNA sequences ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mustela vison deposited as mink

Morphology: epithelial


Source: Organ: uterus

Tissue: endometrium

Cellular Products: alkaline phosphatase [55514]

cytokeratin [55514]

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. GMMe [EPI]细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: June 13, 1994

Age: *****

Gender: female

Comments: The GMMe (ATCC CRL-2674) and GMMs (ATCC CRL-2675) cell lines were established by stable transfection of endometrial tissue using a plasmid vector, pBAPSV40TtsA58, encoding the SV40 large T antigen driven by the human beta-actin promoter. [55514]

The cells were cotransfected with a second plasmid vector, pSV2neo, to confer neomycin resistance. Transfected cells were selected in medium containing G418. [55514]

The cuboidal GMMe cells exhibit epithelial characteristics and the fibroblast GMMs cells exhibit characteristics consistent with a stromal origin. [55514]

GMMe [EPI]细胞The epithelial cells are strongly positive for cytokeratin and weakly positive for vimentin whereas the fibroblast cells are positive for vimentin and negative for cytokeratin. Both lines are negative for desmin and positive for alkaline phosphatase. [55514]

These cell lines are used in coculture with mink embryos in obligate diapause to enhance the length and frequency of embryo survival in vitro. [55514]

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37�C to facilitate dispersal.

GMMe [EPI]细胞Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37�C.


Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006

recommended serum:ATCC 30-2020

References: 55514: Moreau GM, et al. GMMe [EPI]细胞Development of immortalized endometrial epithelial and stromal cell lines from the mink (Mustela vison) uterus and their effects on the survival in vitro of mink blastocysts in obligate diapause. Biol. Reprod. 53: 511-518, 1995. PubMed: 7578673

关于我们| 易展动态| 易展荣誉| 易展服务| 易家文化| 英才集结号| 社会责任| 联系我们

备案号:粤ICP备11010883号| 公安机关备案号:44040202000312| 版权问题及信息删除: 0756-2183610  QQ: 服务QQ

Copyright?2004-2017  珠海市金信桥网络科技有限公司 版权所有

行业网站百强奖牌 搜索营销*有价值奖 中小企业电子商务**服务商
以上信息由企业自行提供,信息内容的真实性、准确性和合法性由相关企业负责,易展仪表展览网对此不承担任何保证责任。

沪公网安备 31011702004356号