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2G3细胞

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  • 产品名称:2G3细胞
  • 产品型号:2G3
  • 产品展商:HZbscience
  • 产品文档:无相关文档
  • 发布时间:2018-09-02
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简单介绍
2G3细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。2G3细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。
产品描述

2G3细胞

数量: 大量

细胞形态: **样

**类型: IgG1

是否是肿瘤细胞: 0

物种来源: 小鼠

生长状态: 混合型生长

运输方式: 冻存运输

ATCC Number: CRL-2435™

Designations: 2G3

Depositors: PJ Bjorkman

Isotype: IgG1

2G3细胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: mixed: adherent and suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against FcRn heavy chain heterodimers

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: 2G3细胞The cell line was established by Louis Gastinel and Pamela Bjorkman in 1992.

Animals were immunized with and twice boosted with soluble neonatal Fc receptor (FcRn) in an adjuvant. Three days before fusion one mouse was boosted with purified FcRn.

Spleen cells were fused with HL-1 Friendly myeloma cells (a derivative of P3X63Ag8.653 that is deficient in both hypoxanthine phosphoribosyl transferase [HPRT] and adenine phosphoribosyl transferase [APRT]).

The antibody recognizes FcRn heavy chain heterodimers.

FcRn is a heterodimer composed of a membrane bound heavy chain attached noncovalently to beta 2-microgloublin. It is structurally similar to class I major histocompatibility (MHC) molecules.

The IG3 (CRL-2434) antibody recognizes two lipid linked forms of FcRn.

These forms are truncated FcRn heavy chain paired with beta 2-microgloublin-DAF (FcRn beta-2 microglobulin DAF) and lipid-linked FcRn paired with unaltered beta 2-microgloublin2G3细胞 (FcRn-DAF/beta 2-microgloublin).

It also recognizes the FcRn-DAF construct.

The 2G3 (CRL-2435) antibody fails to recognize the FcRn heterodimer form in which the heavy chain is attached to the lipid anchor (FcRn-DAF/beta 2-microgloublin) and it also fails to recognize the FcRn-DAF construct.

Unlike 4C9 (CRL-2437), the monoclonal antibodies IG3 (CRL-2434)and 2G3 (CRL-2435) do not recognize rat beta 2-microglobulin alone or complexed with FcRn heavy chains.

This antibody is used in studies of the MHC class I heavy chain FcRn heterodimers and their interaction with IgG. [39787]

Propagation: 2G3细胞ATCC complete growth medium: HL-1 medium supplemented with 4 mM L-glutamine, 1 mM sodium pyruvate and 1% fetal bovine serum. HL-1 medium can be obtained from from Lonza (catalog number 77201).

Temperature: 37.0°C

Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Subcultures are prepared by scraping. Dislodge cells from the flask substrate with a cell scraper. Aspirate gently and dispense into new flasks. Seed flasks at 2 X 10exp5 viable cells/ml.

Preservation: HL-1 Complete Serum-free Medium, 75%; fetal bovine serum, 20%; DMSO, 5%.

References: 39787: Raghavan M, et al. Investigation of the interaction between the class I MHC-related Fc receptor and its immunoglobulin G ligand. Immunity 1: 303-315, 1994. 2G3细胞PubMed: 7889418

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