LYK-1细胞
运输方式： 冻存运输
生长状态： 悬浮生长
是否是肿瘤细胞： 0
物种来源： 大鼠
ATCC Number： HB-306™
细胞形态： **样
**类型： IgG1
数量： 大量
Designations: LYK-1
LYK-1细胞Depositors: Y Yamashita, KS Hathcock
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:LYK-1细胞 immunoglobulin; monoclonal antibody; against a determinant on murine CD44 isoforms expressing variable exon V10.
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Comments: Animals were immunized with a fusion protein corresponding to the products of CD44 variable exons V5 through V10.
Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells.
The antibody is specific for a determinant on murine CD44 isoforms expressing variable exon V10.
Propagation: LYK-1细胞ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate and supplemented with:0.05 mM 2-mercaptoethanol1% Origen Hybridoma Cloning Factor10% fetal bovine serum
Temperature: 37.0°C
Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cultures at cell concentrations between 1 X 10(5) and 8 X 10(5) viable cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
References: 38505: Yamashita Y, et al. LYK-1细胞Tissue-specific and growth-regulated expression of CD44 variable exon determinants in the mouse. Cell. Immunol. 176: 22-33, 1997. PubMed: 9070314
