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28-5-15细胞

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  • 产品名称:28-5-15细胞
  • 产品型号:28-5-15
  • 产品展商:HZbscience
  • 产品文档:无相关文档
  • 发布时间:2018-09-03
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简单介绍
28-5-15细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。28-5-15细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。
产品描述

28-5-15细胞

是否是肿瘤细胞: 0

物种来源: 大鼠

ATCC Number: CRL-2490™

数量: 大量

生长状态: 悬浮生长

细胞形态: **样

**类型: rat IgG2a lambda

运输方式: 冻存运输

Designations: 28-5-15

28-5-15细胞Depositors: DG Sieckmann

Isotype: rat IgG2a lambda

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)

Morphology: lymphoblast


Source: Cell Type:28-5-15细胞 hybridoma:lymphoblast B lymphocyte; somatic cell hybrid

Cellular Products: immunoglobulin; monoclonal antibody; against MOPC167 idiotype (VH1/V kappa 24)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: This cell line was deposited at the ATCC by Donna G. Sieckmann, Naval Medical Research Center. The line is distributed for academic research purposes only. The Naval Medical Research Center releases the line subject to the following: 1.) The line or its products must not be distributed to third parties. Commercial interests are the exclusive property of the Naval Medical Research Center. 2.) Any proposed commercial use of the cell line including any use by a for-profit entity must first be negotiated with the Commanding Officer, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910-7500.

Isolation: Isolation date: 1990

Comments: Animals were immunized with MOPC167 and HPCM27 anti-phosphocholine (PC) antibodies. MOPC167 is an IgA myeloma specific for PC and HPCM27 is an IgM myeloma specific for PC.

28-5-15细胞Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells.

The antibody binds the VH1/V kappa 24 heavy and light chain idiotypic specificities of the phosphocholine specific myeloma protein, MOPC167.

It completely inhibits the binding of MOPC167 to phosphocholine. It can bind the membrane receptor of 207-4 transgenic B cells and induce proliferation of these B cells.

The antibody does not bind VH1/V kappa 22, VH1/V kappa 8, or VH1/V kappa 1 PC-binding proteins or other IgA or IgM myeloma proteins. [40008]

This antibody can be used as a typing reagent for mouse Ig bearing the VH1/V kappa 24 idiotypic specificity and can be used in typing the 207-4 immunoglobulin transgenic mice that carry the VH1/V kappa 24 gene segment of MOPC167 myeloma.

Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 85%; fetal bovine serum, 15%

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10(4) viable cells/ml. Do not allow the cell concentration to exceed 5 X 10(5) cells/ml.

Interval: Maintain cultures at a cell concentration between 5 X 10(4) and 5 X 10(5) cells/ml.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: 28-5-15细胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005

recommended serum:ATCC 30-2020

Cell culture tested DMSO:ATCC 4-X

Erythrosin B vital stain solution:ATCC 30-2404

Trypan Blue vital stain solution:ATCC 30-2402

References: 40008: Sieckmann DG, et al. Anti-idiotype monoclonal antibodies specific for the MOPC167 anti-phosphocholine transgene-encoded antibody. Hybridoma 16: 503-511, 1997. PubMed: 9455702

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