28-5-15细胞
是否是肿瘤细胞： 0
物种来源： 大鼠
ATCC Number： CRL-2490™
数量： 大量
生长状态： 悬浮生长
细胞形态： **样
**类型： rat IgG2a lambda
运输方式： 冻存运输
Designations: 28-5-15
28-5-15细胞Depositors: DG Sieckmann
Isotype: rat IgG2a lambda
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type:28-5-15细胞 hybridoma:lymphoblast B lymphocyte; somatic cell hybrid
Cellular Products: immunoglobulin; monoclonal antibody; against MOPC167 idiotype (VH1/V kappa 24)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions: This cell line was deposited at the ATCC by Donna G. Sieckmann, Naval Medical Research Center. The line is distributed for academic research purposes only. The Naval Medical Research Center releases the line subject to the following: 1.) The line or its products must not be distributed to third parties. Commercial interests are the exclusive property of the Naval Medical Research Center. 2.) Any proposed commercial use of the cell line including any use by a for-profit entity must first be negotiated with the Commanding Officer, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910-7500.
Isolation: Isolation date: 1990
Comments: Animals were immunized with MOPC167 and HPCM27 anti-phosphocholine (PC) antibodies. MOPC167 is an IgA myeloma specific for PC and HPCM27 is an IgM myeloma specific for PC.
28-5-15细胞Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells.
The antibody binds the VH1/V kappa 24 heavy and light chain idiotypic specificities of the phosphocholine specific myeloma protein, MOPC167.
It completely inhibits the binding of MOPC167 to phosphocholine. It can bind the membrane receptor of 207-4 transgenic B cells and induce proliferation of these B cells.
The antibody does not bind VH1/V kappa 22, VH1/V kappa 8, or VH1/V kappa 1 PC-binding proteins or other IgA or IgM myeloma proteins. [40008]
This antibody can be used as a typing reagent for mouse Ig bearing the VH1/V kappa 24 idiotypic specificity and can be used in typing the 207-4 immunoglobulin transgenic mice that carry the VH1/V kappa 24 gene segment of MOPC167 myeloma.
Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 85%; fetal bovine serum, 15%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10(4) viable cells/ml. Do not allow the cell concentration to exceed 5 X 10(5) cells/ml.
Interval: Maintain cultures at a cell concentration between 5 X 10(4) and 5 X 10(5) cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: 28-5-15细胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
recommended serum:ATCC 30-2020
Cell culture tested DMSO:ATCC 4-X
Erythrosin B vital stain solution:ATCC 30-2404
Trypan Blue vital stain solution:ATCC 30-2402
References: 40008: Sieckmann DG, et al. Anti-idiotype monoclonal antibodies specific for the MOPC167 anti-phosphocholine transgene-encoded antibody. Hybridoma 16: 503-511, 1997. PubMed: 9455702
