TR 310细胞
生长状态: 悬浮生长
ATCC Number: HB-219™
运输方式: 冻存运输
数量: 大量
是否是肿瘤细胞: 0
物种来源: 大鼠
细胞形态: **样
**类型: IgG2b
Designations: TR 310
TR 310细胞Depositors: IL Weissman
Isotype: IgG2b
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: TR 310细胞immunoglobulin; monoclonal antibody; against mouse T cell receptor
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Comments: Animals were immunized with the Tk1 murine T cell lymphoma.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
TR 310细胞The antibody reacts with a deteminant on the mouse T cell antigen receptor.
It recognizes a determinant encoded by the Vb7 (V beta 7) gene segment.
Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine, 1 mM sodium pyruvate and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. TR 310细胞Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.