Lu Rob细胞
ATCC Number： CRL-1397™
相关**： 其他**
细胞形态： 成纤维样
年限： 50 years
器官来源： 皮肤
是否是肿瘤细胞： 0
物种来源： 人
运输方式： 冻存运输
Lu Rob细胞数量： 大量
生长状态： 贴壁生长
Designations: Lu Rob
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: skin
Disease: Lu Rob细胞Ehlers-Danlos syndrome, type IV
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 50 years
Gender: female
Comments: The tissue donor suffered from type IV Ehlers-Danlos syndrome.
Propagation: Lu Rob细胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove spent medium, add fresh 0.25% trypsin for 3 to 5 minutes, remove trypsin and let the culture sit at 37C for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Lu Rob细胞Subculture every 6 to 8 days.
