GeLu细胞
器官来源： 肺
细胞形态： 成纤维样
数量： 大量
运输方式： 冻存运输
生长状态： 贴壁生长
年限： 403 days
是否是肿瘤细胞： 0
物种来源： 其他
GeLu细胞ATCC Number： CCL-100™
Designations: GeLu
Depositors: ML Macy
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Meriones unguiculatus
Morphology: fibroblast
Source: Organ: lung
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. GeLu细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Virus Resistance: poliovirus 1, 3
Age: 403 days
Gender: female
Propagation: ATCC complete growth medium: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, rinse cell sheet twice with 0.25% trypsin - 0.53 mM EDTA solution and remove. Place culture at room temperature until cells detach (approximately 10 to 20 minutes). Add fresh medium, aspirate and dispense into new flasks.
GeLu细胞Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
References: 26276: . . TCA GeLu细胞(Tissue Cult. Assoc.) Man. 3: 617-621, 1977.
