Ltk-11细胞
生长状态： 贴壁生长
细胞形态： 成纤维样
是否是肿瘤细胞： 0
物种来源： 小鼠
品系： C3H/An
年限： 100 days *****
组织来源： subcutaneous connective tissue; areolar and adipose
数量： 大量
运输方式： 冻存运输
Ltk-11细胞ATCC Number： CRL-10422™
相关**： 正常
Designations: Ltk-11
Depositors: Synaptic Pharmaceutical Corporation
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus
Morphology: fibroblast
Source: Tissue: subcutaneous connective tissue; areolar and adipose
Strain: C3H/An
Disease: normal
Ltk-11细胞Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors: 5-hydroxytryptamine (serotonin) 1D beta (5HT1Dbeta)
Age: 100 days *****
Gender: male
Comments: This line is a derivative of L-M(TK-) (see ATCC CCL-1.3) that is stably transfected with the gene for the human 5HT1Dbeta receptor.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing:Ltk-11细胞 Protocol: Remove old medium, add fresh 0.25% trypsin, EDTA (0.02%) solution, rinse and remove. Allow cells to sit at room temperature (or at 37C) until cells detach (approximately 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Twice per week
Preservation: Freeze medium: Complete growth medium, 90%; DMSO, 10%
Storage temperature: liquid nitrogen vapor phase
Related Products: parental cell line:ATCC CCL-1.3
References: 90271: Ltk-11细胞Konkel M, et al. Compounds specific for the human .alpha.1d adrenergic receptor and uses thereof. U.S. Patent 6,706,716 dated Mar 16 2004
