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LS132.1D9 [1D9]细胞

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  • 产品名称:LS132.1D9 [1D9]细胞
  • 产品型号:LS132.1D9 [1D9]
  • 产品展商:HZbscience
  • 产品文档:无相关文档
  • 发布时间:2018-07-20
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简单介绍
LS132.1D9 [1D9]细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。LS132.1D9 [1D9]细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。
产品描述

LS132.1D9 [1D9]细胞

生长状态: 悬浮生长

ATCC Number: HB-12549™

是否是肿瘤细胞: 0

物种来源: 小鼠

运输方式: 冻存运输

数量: 大量

**类型: IgG2a kappa

Designations: LS132.1D9 [1D9]

LS132.1D9 [1D9]细胞Depositors: LeukoSite, Inc.

Isotype: IgG2a kappa

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma)

Morphology:

Source: Cell Type: hybridoma:

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material.LS132.1D9 [1D9]细胞 Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with CCR2bDEF3.L1/2 cells derived from the murine pre-B lymphoma cell line, L1/2, transfected with the human CCR2B cDNA coding region. Spleen cells were fused Sp2/0 myeloma cells. The antibody binds to the human CC-chemokine receptor 2 (CCR2).

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 25 mM HEPES, 20 pg/ml mouse recombinant Interleukin-6 (IL-6) and 20% fetal bovine serum

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml. Do not allow the cell concentration to exceed 1 X 10(6) cells/ml.

Interval: LS132.1D9 [1D9]细胞Maintain cultures at a cell concentration between 1 X 10(5) and 1 X 10(6) cells/ml.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 71858: LaRosa GJ. Anti-CCR2 antibodies and methods of use therefor. US Patent 6,312,689 dated Nov 6 2001

71859: LaRosa GJ, et al. Anti-CCR2 antibodies and methods of use therefor. US Patent 6,352,832 dated Mar 5 2002

71860: LaRosa GJ. Method of inhibiting leukocyte trafficking by anti-CCR2 amino-terminal domain antibodies. US Patent 6,395,497 dated May 28 2002

71861: LaRosa GJ. Method of inhibiting interaction of cells bearing CCR2 by Anti-CCR2 amino-terminal domain antibodies. US Patent 6,406,865 dated Jun 18 2002

71862: LaRosa GJ. Anti-CCR2 antibodies. US Patent 6,406,694 dated Jun 18 2002

71863: LaRosa GJ. Method of inhibiting cell function associated with CCR2 by anti-CCR2 amino-terminal domain antibodies. US Patent 6,448,021 dated Sep 10 2002

71864: LaRosa GJ. LS132.1D9 [1D9]细胞Anti-CCR2 antibodies and methods of use therefor. US Patent 6,451,522 dated Sep 17 2002

71865: . Anti-CCR2 antibodies and methods of use therefor. US Patent 6,458,353 dated Oct 1 2002

71866: LaRosa GJ. Anti-CCR2 antibodies and methods of use therefor. US Patent 6,491,915 dated Dec 10 2002

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