TM1细胞
细胞形态： **样
**类型： IgM
数量： 大量
生长状态： 悬浮生长
运输方式： 冻存运输
是否是肿瘤细胞： 0
物种来源： 小鼠
ATCC Number： HB-169™
Designations: TM1
TM1细胞Depositors: F Grumet
Isotype: IgM
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against Leu-5; cytotoxic
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. TM1细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with human leukocytes (HLA phenotype A2,A28,Bw4,B27,B44,Cw2).
Spleen and lymph node cells were fused with Sp2/08A2 myeloma cells.
The antibody is cytotoxic for Leu-5 positive (Leu-5+) cells.
Tested and found negative for ectromelia virus (mousepox).
TM1细胞Propagation: ATCC complete growth medium: Modified RPMI 1640 medium (see fact sheet), 90%; fetal bovine serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1173: Grumet FC, et al. TM1细胞A simplified new method for HLA-DR typing using the TM1 monoclonal antibody. Hum. Immunol. 6: 63-73, 1983. PubMed: 6601099
