GAP A3细胞
细胞形态： **样
**类型： IgG2a; kappa light chain
运输方式： 冻存运输
是否是肿瘤细胞： 0
物种来源： 小鼠
生长状态： 悬浮生长
ATCC Number： HB-122™
数量： 大量
GAP A3细胞Designations: GAP A3
Depositors: AE Berger
Isotype: IgG2a; kappa light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension, suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against HLA A3
Permits/Forms: GAP A3细胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with human peripheral blood lymphocytes.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody does not react with HLA A11.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
fetal bovine serum to a final concentration of 10%
1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
Subculturing: GAP A3细胞Protocol: Cultures can be maintained by addition or replacement of medium. Establish new cultures at 1 X 10(5) viable cells/ml and maintain between 2 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: recommended serum:ATCC 30-2020
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
Cell culture tested DMSO:ATCC 4-X
References: 22280: . . Fed. Proc. 42: 1371, 1983.
58007: Dieckmann CL, et al. Monoclonal antibody to HLA-A3. Hybridoma 1: 87-90, 1982.GAP A3细胞 PubMed: 6208124
