GAP 8.3细胞
ATCC Number： HB-12™
运输方式： 冻存运输
数量： 大量
是否是肿瘤细胞： 0
物种来源： 小鼠
细胞形态： **样
**类型： IgG2a; kappa light chain
生长状态： 悬浮生长
Designations: GAP 8.3
GAP 8.3细胞Depositors: AE Berger
Isotype: IgG2a; kappa light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against human leukocyte (monocytes, lymphocytes, granulocytes); against CD45
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Comments: Mice were immunized with MOLT-4 cells a human T cell line (MOLT-4, ATCC CRL-1582).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody recognizes a 200000 dalton antigen analogous to the mouse T200 antigen and the rat common leukocyte antigen (it does not react with erythrocytes).
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
GAP 8.3细胞Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 914: Berger AE, et al. GAP 8.3细胞A human leukocyte antigen identified by a monoclonal antibody. Hum. Immunol. 3: 231-245, 1981. PubMed: 6975769
