TIMI.4G.1.3细胞
细胞类型: T**细胞
数量: 大量
是否是肿瘤细胞: 0
物种来源: 小鼠
运输方式: 冻存运输
细胞形态: **样
品系: C57BL/6
ATCC Number: TIB-38™
相关**: **瘤
TIMI.4G.1.3细胞生长状态: 悬浮生长
Designations: TIMI.4G.1.3
Depositors: R Hyman
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus
Morphology: lymphoblast
Source: Disease: lymphoma
Strain: C57BL/6
Cell Type: TIMI.4G.1.3细胞T lymphocyte;
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Antigen Expression: H-2b; Thy-1.2
Comments: This line was derived from TIMI.4 (ATCC TIB-37).
The cells are resistant to 0.1 mM 6-thioguanine.
Tested and found negative for ectromelia virus (mousepox).
Propagation: TIMI.4G.1.3细胞ATCC complete growth medium: Dulbecco's modified Eagle's medium, 90%; horse serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1129: Hyman R. Studies on surface antigen variants. TIMI.4G.1.3细胞Isolation of two complementary variants for Thy 1.2. J. Natl. Cancer Inst. 50: 415-422, 1973. PubMed: 4702114