LP3IIG2细胞
ATCC Number： HB-8472™
是否是肿瘤细胞： 0
物种来源： 小鼠
数量： 大量
生长状态： 悬浮生长
运输方式： 冻存运输
细胞形态： **样
**类型： IgG2a
LP3IIG2细胞Designations: LP3IIG2
Depositors: Genetic Systems Corp.
Isotype: IgG2a
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: LP3IIG2细胞immunoglobulin; monoclonal antibody; against Legionella pneumophila
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Comments: Animals were immunized with Legionella pneumophila.
Spleen cells were fused with NS-1 myeloma cells.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
LP3IIG2细胞Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 5 X 10 exp5 cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 3893: Goldstein LC, Gosting LH. LP3IIG2细胞Method and composition for the detection and diagnosis of Legionella pneumophila. US Patent 4,851,333 dated Jul 25 1989
