Gam Per细胞
数量： 大量
器官来源： 皮肤
年限： 63 years
ATCC Number： CRL-1326™
相关**： Ehlers-Danlos综合征子
生长状态： 贴壁生长
运输方式： 冻存运输
是否是肿瘤细胞： 0
物种来源： 人
细胞形态： 成纤维样
Gam Per细胞Designations: Gam Per
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: skin
Disease: Ehlers-Danlos syndrome, possible heterozygote
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Gam Per细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 63 years
Gender: female
Comments: The tissue donor was a possible heterozygote for Ehlers-Danlos syndrome, and was the grandmother of Jo Per (ATCC CRL-1332), No Per (ATCC CRL-1327) and Cor Per II (ATCC CRL-1243).
Gam Per细胞Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 2 to 3 times per week
Remove spent medium, add fresh 0.25% trypsin for 3 to 5 minutes, remove trypsin and let the culture sit at 37C for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Gam Per细胞Subculture every 8 to 10 days.
