2A3A1H细胞
细胞形态： **样
**类型： IgG1
生长状态： 悬浮生长
运输方式： 冻存运输
数量： 大量
是否是肿瘤细胞： 0
物种来源： 小鼠
ATCC Number： HB-8555™
2A3A1H细胞Designations: 2A3A1H
Depositors: Immunex Corporation
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:2A3A1H细胞 immunoglobulin; monoclonal antibody; against human interleukin 2 (interleukin-2, IL-2) receptor
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Comments: Animals were immunized with phytohemagglutinin activated human peripheral blood (PBL) leukocytes.
Spleen cells were fused with NS-1 myeloma cells.
The antibody binds to IL-2 receptors on human leukocytes and blocks both mitogen induced and antigen induced PBL proliferation.
Propagation: 2A3A1H细胞ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.025 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 6 X 10 exp5 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
References: 3994: March CJ, et al. Hybridoma antibody which binds IL-2 receptor. US Patent 4,845,198 dated Jul 4 1989
3995: March CJ, et al. Interleukin-2 receptor. 2A3A1H细胞US Patent 4,578,335 dated Mar 25 1986
