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Lp1 MAB 2细胞

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  • 产品名称:Lp1 MAB 2细胞
  • 产品型号:Lp1 MAB 2
  • 产品展商:HZbscience
  • 产品文档:无相关文档
  • 发布时间:2018-08-23
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简单介绍
Lp1 MAB 2细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。Lp1 MAB 2细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。
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Lp1 MAB 2细胞

生长状态: 悬浮生长

是否是肿瘤细胞: 0

物种来源: 小鼠

细胞形态: **样

**类型: IgG2b

ATCC Number: CRL-1770™

运输方式: 冻存运输

数量: 大量

Designations: Lp1 MAB 2

Lp1 MAB 2细胞Depositors: RM McKinney

Isotype: IgG2b

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against Legionella pneumophila serogroup 1

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Lp1 MAB 2细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with the bacterial antigenic subgroup Knoxville 1 (ATCC 33153).

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

The antibody reacts with a surface antigen on L. pneumophila and is useful in clinical diagnostic tests for subtyping L. pneumophila serogroup 1 isolates for epidemiologic studies using indirect immunofluorescence assays.

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. Lp1 MAB 2细胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.

Subculturing: Medium Renewal: Every 2 to 4 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 1190: Joly JR, et al. Development of a standard subgrouping scheme for Legionella pneumophila serogroup 1 using monoclonal antibodies. J. Clin. Microbiol. 23: 768-771, 1986. PubMed: 3517064

58069: McKinney RM, et al. Monoclonal antibodies to Legionella pneumophila serogroup 1: possible applications in diagnostic tests and epidemiologic studies. Zentralbl. Bakteriol. Mikrobiol. Hyg. I Abt. Lp1 MAB 2细胞Orig. A255: 91-95, 1983.

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