12A5B7细胞
生长状态： 悬浮生长
细胞形态： **样
**类型： IgG
数量： 大量
ATCC Number： HB-8328™
运输方式： 冻存运输
是否是肿瘤细胞： 0
物种来源： 小鼠
12A5B7细胞Designations: 12A5B7
Depositors: Immunomedics, Inc.
Isotype: IgG
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: 12A5B7细胞immunoglobulin; monoclonal antibody; against human complement C1q
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Comments: Animals were immunized with purified human C1q.
12A5B7细胞Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 3547: Reckel RP, et al. 12A5B7细胞Method for detecting immune complexes in serum. US Patent 4,595,654 dated Jun 17 1986
