G2a.5细胞
数量： 大量
是否是肿瘤细胞： 0
物种来源： 小鼠
ATCC Number： HB-110™
生长状态： 悬浮生长
运输方式： 冻存运输
细胞形态： **样
**类型： IgG2a
Designations: G2a.5
G2a.5细胞Depositors: P Parham
Isotype: IgG2a
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against HLA DC1 (HLA DR)
Permits/Forms: G2a.5细胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with glycoprotein fraction from the Bristol 8 human B lymphoblastoid cell line.
Spleen cells were fused with NS-1 myeloma cells.
This line is a switch variant of Genox 3.53 (ATCC HB-103).
An IgG2b switch variant of Genox 3.53 also exists (G2b.2, ATCC HB-109).
G2a.5细胞Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: RPMI 1640 medium with 0.1 mM hypoxanthine, 90%; fetal bovine serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 994: Parham P, et al. Isolation of heavy chain class switch variants of a monoclonal anti-DC1 hybridoma cell line: effective conversion of noncytotoxic IgG1 antibodies to antibodies. Hum. Immunol. 8: 141-151, 1983. G2a.5细胞PubMed: 6417068
