TH-1, Subline B1细胞
数量： 大量
细胞形态： 上皮样
ATCC Number： CCL-50™
相关**： 正常
生长状态： 贴壁生长
运输方式： 冻存运输
器官来源： 心脏
是否是肿瘤细胞： 0
TH-1, Subline B1细胞物种来源： 其他
Designations: TH-1, Subline B1
Depositors: HF Clark
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Terrapene carolina
Morphology: epithelial
Source: Organ: heart
Disease: TH-1, Subline B1细胞normal
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions: This material is not available for international distribution due to the lack of historical data to support the issuance of a U.S. Fish and Wildlife Service Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES) export permit
Virus Resistance: poliovirus 1
Comments: Growth is inhibited at alkaline pH. Medium should be adjusted to neutrality after plating.
Propagation: TH-1, Subline B1细胞ATCC complete growth medium: Basal medium (Eagle) in Hanks' BSS, 90%; fetal bovine serum, 10%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: Every 10 to 14 days
Remove medium, add fresh 0.25% trypsin for 2 to 3 minutes, remove trypsin and allow the culture to stand for 5 to 10 minutes at room temperature. Add fresh medium, aspirate and dispense into new flasks.
References: 877: Clark HF, Karzon DT. Acquired tolerance to elevated temperatures in a poikilothermic cell line (terrapene heart, TH-1). Exp. Cell Res. 48: 269-275, 1967. PubMed: 6082311
878: Clark HF, Karzon DT. Terrapene heart (TH-1), a continuous cell line from the heart of the box turtle Terrapene carolina. Exp. Cell Res. 48: 263-268, 1967. PubMed: 4295486
57990: Huang CC, Clark HF. Chromosome changes in cell lines of the box turtle (Terrapene carolina) grown at two different temperatures. Can. J. Genet. Cytol. 9: 449-461, 1967. TH-1, Subline B1细胞PubMed: 6082587
